Determination of Organic Phosphates in Rat Heart Muscle with an Organic Phosphates Analyzer.
نویسندگان
چکیده
منابع مشابه
Hemoglobin kinetics and the effect of organic phosphates.
A kinetic model based on allosteric mechanisms of cooperativity fits the experimentally observed phosphate dependence of hemoglobin reactions. Subunit inequivalence is found to be important in analyzing hemoglobin kinetics. The observed increase in the rate of deoxygenation in the presence of organic phosphates is primarily related to the increased rate of dissociation of the second oxygen mole...
متن کاملEffect of organic phosphates on methemoglobin reduction by ascorbic acid.
The rate of methemoglobin reduction by ascorbic acid was accelerated in the presence of ATP,2,3-diphosphoglycerate (2,3-DPG), and inositol hexaphosphate (IHP). The acceleration was as much as three times, four times, and ten times in the presence of ATP, 2.3-DPG, and IHP at pH 7.0, respectively. The changes of the concentrations of methemoglobin and ascorbic acid during the methemoglobin reduct...
متن کاملHyperpolarised organic phosphates as NMR reporters of compartmental pH.
Organic phosphate metabolites contain functional groups with pKa values near the physiologic pH range, yielding pH-dependent (13)C chemical shift changes of adjacent quaternary carbon sites. When formed in defined cellular compartments from exogenous hyperpolarised (13)C substrates, metabolites can thus yield localised pH values and correlations of organelle pH and catalytic activity.
متن کاملA robotics-based automated assay for inorganic and organic phosphates.
Phosphate analyses are fundamental to a broad range of biochemical applications involving inorganic phosphate and organic phosphoesters such as phospholipids, phosphorylated proteins, and nucleic acids. A practical automated method utilizing robotics is described in this report. Five colorimetric methods of phosphate analyses based on formation of a phosphomolybdate complex and compatible with ...
متن کاملThe interaction between erythrocyte organic phosphates, magnesium ion, and hemoglobin.
Oxygen equilibria have been used to study the interaction between red cell organic phosphates, magnesium ion, and hemoglobin. We measured the oxygen affinity of hemoglobin solutions containing either 2,3-diphosphoglycerate (2,3-DPG) or ATP and varying concentrations of magnesium ion at lo”, ZO”, and 30”. Deoxyhemoglobin and Mg2f were competitors, binding reciprocally with the organic phosphates...
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ژورنال
عنوان ژورنال: Analytical Sciences
سال: 1997
ISSN: 0910-6340,1348-2246
DOI: 10.2116/analsci.13.601